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KMID : 1177720130070010011
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Journal of Alternatives to Animal Experiments
2013 Volume.7 No. 1 p.11 ~ p.16
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Phototoxicity Study of BTT0811 using in vitro 3T3 Neutral Red Uptake Assay
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Jeon Eun-Young
Jung Mi-Sook
Seo Im-Kwon
Noh Hyo-Jeong
Jung Woo-Young
Kim Sang-Seok
Bae Heung-Mo
Jeon Tae-Won
Lee Sang-Goo
Kang Jong-Koo
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Abstract
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Phototoxicity is a toxicity reaction induced by UV after applying the chemical to part or entire body. BTT0811 which has co-developed by Biotoxtech Co., Ltd., Korea and Northland, China is recombinant material of thymosin β4. We performed to evaluate the phototoxicity of BTT0811 using in vitro 3T3 neutral red uptake phototoxicity test in OECD guideline. Before evaluating the phototoxicity of this study, we selected two substances for the controls. One of the positive controls was chlorpromazine, already known as a phototoxicity substance, and the other was L-histidine, which has no phototoxicity. Then, we evaluated the phototoxicity of BTT0811 compared with those substances. In results of study, photo irritation factor (PIF) and mean photo effect (MPE) was measured for evaluating phototoxicity. PIF and MPE value of chlorpromazine was more than 6 and 0.15, respectively. Chlorpromazine had phototoxicity resulting from PIF and MPE. On the other hand, L-histidine was measured no PIF value and less than 0.1 of MPE. There was no PIF value of BTT0811 and MPE value of BTT0811 was less than 0.1. Based on the results of this study, BTT0811 did not show any evidence of phototoxicity in BALB/c 3T3 clone A31 cells under the conditions of this study.
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KEYWORD
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BTT0811, Alternative Test Method, in vitro 3T3, Neutral Red Uptake, Phototoxicity Test, Phototoxicity
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